Jo-Wilfried Tsonga became the latest seed to lose at the Aegon Championships, beaten in straight sets by Luxembourg’s Gilles Muller.
The Frenchman, seeded fifth, lost 6-4 6-4 in the second round at Queen’s.
Tsonga’s exit follows defeats for Andy Murray, Stan Wawrinka and Milos Raonic – the top three seeds – on Tuesday.
Croatia’s fourth seed Marin Cilic and Bulgarian sixth seed Grigor Dimitrov are the leading players left in the draw at the London tournament.
Also read: Andy Murray confident of defending Wimbledon after shocking exit from Aegon Championships
Muller needed just one hour and nine minutes to eliminate Tsonga, seeded No.5, and advance to the quarterfinals.
Tsonga’s loss to 34-year-old Muller was the most recent upset in the tournament this year after defending champion Andy Murray suffered a shock first round defeat on Tuesday.
Murray, World No.1, was defeated by Australian Jordan Thompson after Swiss Stan Wawrinka and Canadian Milos Raonic, second and third seed, respectively, were knocked out.
Croatian Marin Cilic is the only player remaining from the top five seeded players in the preparatory event for Wimbledon, which starts on July 3.
With inputs from agencies.